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diisothio cyanostilbene  (Tocris)


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    Tocris diisothio cyanostilbene
    Diisothio Cyanostilbene, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/diisothio+cyanostilbene/DIDS/pm41955096-358-44-54
    Average 94 stars, based on 53 article reviews
    diisothio cyanostilbene - by Bioz Stars, 2026-11
    94/100 stars

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    Western Blot:

    Article Title: BEST1-mediated tonic excitation facilitates anxiety-like behaviors in a mouse model of chronic neuropathic pain.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFAP (IF, 1:1000) Millipore Cat# AB5541; RRID: AB_177521 Chicken anti-GFP (IF, 1:1000) Millipore Cat# AB16901; RRID: AB_90890 Rabbit anti-BEST1 (WB, 1:2000; IF, 1:200) Cohesion Cat# CPA9240 Rabbit anti-BEST1 (IF, 1:100) Abcam Cat# ab14927; RRID: AB_301518 Rabbit anti-β-actin (WB, 1:6000) Bioss Cat# bs-0061R; RRID: AB_10855480 Rabbit anti-β-tubulin (WB, 1:6000) Bioworld Technology Cat# BS1482M; RRID:AB_3068320 Rabbit anti-GluA1 (WB,1:2000) Abcam Cat# ab109450; RRID: AB_10860361 Rabbit anti-GluA2 (WB, 1:2000 Abcam Cat# ab133477; RRID: AB_2620181 Rabbit anti-nNOS (WB, 1:2000; IF, 1:400) Thermo Fisher Scientific Cat# 61–7000; RRID: AB_2313734 Rabbit anti-stargazin (WB, 1:1000) Millipore Cat# 07–577; RRID: AB_310726 Mouse anti-CaMKIIα (IF, 1:200) Cell Signaling Technology Cat# 50049; RRID: AB_2721906 Mouse anti-GAD67 (IF, 1:200) Abcam Cat# ab26116; RRID: AB_448990 Mouse anti-GAPDH (WB, 1:6000) Kangcheng Cat# KC-5G4; RRID: AB_2493106 Mouse anti-NeuN (IF, 1:500) Millipore Cat# MAB377; RRID: AB_2298772 Goat anti-chicken Alex 488 (IF, 1:400) Jackson ImmunoResearch Cat# 103-545-155; RRID: AB_2337390 Goat anti-rabbit Cy3 (IF, 1:400) Jackson ImmunoResearch Cat# 111-165-003; RRID: AB_2338000 Goat anti-rabbit HRP (WB, 1:12,000) Multi Science Cat# GAR0072; RRID:AB_2827833 Goat anti-mouse Alexa 488 (IF, 1:400) Jackson ImmunoResearch Cat#115-545-003; RRID: AB_2338840 Goat anti-mouse HRP (WB, 1:15,000) Multi Science Cat# GAM0072; RRID: AB_2827834 Bacterial and virus strains AAV-CAG-shBest1-eGFP Obio Technology custom-made AAV-CAG-shScrambled-eGFP Obio Technology custom-made AAV-Syn-Cre-eGFP Obio Technology Cat# H4942 AAV-Syn-eGFP Obio Technology Cat# E1190 AAV-CaMKIIa-GCaMP6s BrainVTA Cat# PT-0110 AAV-CaMKIIα-stargazin(C302S)-3flagT2A-mCherry GeneChem custom-made AAV-CaMKIIα-3flag-T2A-mCherry GeneChem NA AAV-Syn-taCasp3-eGFP BrainVTA Cat# PT-2433 AAV-Syn-eGFP BrainVTA Cat# PT-1990 Chemicals, peptides, and recombinant proteins Isoflurane RWD Life Science Cat# R510-220 Dihydrokainic acid (DHK) Tocris Cat# 0111 Ceftriaxone sodium (CTX) Roche NA L-glutamic acid (glutamate) Sigma-Aldrich Cat# G1251 Hoechst 332588 (HOE) Sigma-Aldrich Cat# B1155 Bicuculline methobromide (BMI) Tocris Cat# 0109 Kynurenic acid (KynA) Sigma-Aldrich Cat# K3375 Sulfasalazine (SAS) Sigma-Aldrich Cat# PHR3243 DL-threo-β-benzyloxyaspartic acid (TBOA) Tocris Cat# 1223 Tetrodotoxin (TTX) Sigma-Aldrich Cat# T8024 D-(− )-2-Amino-5-phosphonopentanoic acid (AP-5) Tocris Cat# 0106 (Continued on next page) 20 Cell Reports 45, 117229, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER 6-cyano-7-nitroquinoxaline-2,3dioneis (CNQX) Sigma-Aldrich Cat# C239 CaCCinh-A01 Tocris Cat# 4877 DCPIB Tocris Cat# 1540 T16Ainh-A01 Tocris Cat# 4538 1,2-bis(2-aminophenoxy)ethaneN,N,N,N-tetraacetic acid (BAPTA) Sigma-Aldrich Cat# A4926 4,4′-Diisothio-cyanostilbene-2,2′disulfonic acid (DIDS) Tocris Cat# 4523 Dil Beyotime Biotechnology Cat# C1036 TEA chloride Sigma-Aldrich Cat# T2265 Critical commercial assays FD rapid GolgiStain kit FD Neuro Technologies Cat# PK401 Deposited data Original western blot images This paper MendeleyData, https://doi.org/10.17632/ 9knfvskjfz.1 Experimental models: Organisms/strains C57BL/6 mice GemPharmatech Co. Ltd Cat# N000013 BestloxP/loxP (C57BL/6JGpt-Best1em1Cflox/ Gpt) mice GemPharmatech Co. Ltd Strain# T017974; RRID: IMSR_GPT:T017974 nNOSloxP/loxP mice A gift from Prof. Qi-Gang Zhou (Nanjing Medical University, China) NA Software and algorithms Adobe Photoshop CS6 Adobe Systems https://www.adobe.com/; RRID: SCR_014199 GraphPad Prism 8.0.2 GraphPad Software, Inc. https://www.graphpad.com/; RRID: SCR_002798 Image-Pro Plus software Media Cybernetics http://www.mediacy.com/imageproplus; RRID:SCR_007369 Imaris 7.5.2 software Oxford Instruments https://imaris.oxinst.cn/products/imaris- for-neuroscientists/; RRID: SCR_007370 pClamp 10.3 Molecular Devices http://mdc.custhelp.com/; RRID: SCR_011323 Mini analysis program 6.0 Synaptosoft, Inc. http://www.synaptosoft.com/ MiniAnalysis/; RRID: SCR_002184 Image Lab (Beta 7) Bio-Rad Laboratories http://www.bio-rad.com/en-us/sku/ 1709690-image-lab-software/; RRID: SCR_014210 ZEN 3.4 Light Edition Zeiss https://www.zeiss.com/; RRID: SCR_ 023747 MATLAB Thinker Tech Nanjing Biotech Ltd. http://www.mathworks.com/products/ matlab/;RRID:SCR_001622 Analyst R914.6.2 AB Sciex https://sciex.com/products/software/ analyst-tf-software; RRID:SCR_015785 TopScan LITE software Clever Sys, Inc. http://www.cleversysinc.com/; RRID: SCR_014494 Python software Python Software Foundation http://www.python.org/; RRID:SCR_008394 Other Osmotic micropump Alzet Model 1007D Enhanced chemiluminescence Millipore Cat #WBKLS0500 Cell Reports 45, 117229, April 28, 2026 21 ..

    Software:

    Article Title: BEST1-mediated tonic excitation facilitates anxiety-like behaviors in a mouse model of chronic neuropathic pain.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFAP (IF, 1:1000) Millipore Cat# AB5541; RRID: AB_177521 Chicken anti-GFP (IF, 1:1000) Millipore Cat# AB16901; RRID: AB_90890 Rabbit anti-BEST1 (WB, 1:2000; IF, 1:200) Cohesion Cat# CPA9240 Rabbit anti-BEST1 (IF, 1:100) Abcam Cat# ab14927; RRID: AB_301518 Rabbit anti-β-actin (WB, 1:6000) Bioss Cat# bs-0061R; RRID: AB_10855480 Rabbit anti-β-tubulin (WB, 1:6000) Bioworld Technology Cat# BS1482M; RRID:AB_3068320 Rabbit anti-GluA1 (WB,1:2000) Abcam Cat# ab109450; RRID: AB_10860361 Rabbit anti-GluA2 (WB, 1:2000 Abcam Cat# ab133477; RRID: AB_2620181 Rabbit anti-nNOS (WB, 1:2000; IF, 1:400) Thermo Fisher Scientific Cat# 61–7000; RRID: AB_2313734 Rabbit anti-stargazin (WB, 1:1000) Millipore Cat# 07–577; RRID: AB_310726 Mouse anti-CaMKIIα (IF, 1:200) Cell Signaling Technology Cat# 50049; RRID: AB_2721906 Mouse anti-GAD67 (IF, 1:200) Abcam Cat# ab26116; RRID: AB_448990 Mouse anti-GAPDH (WB, 1:6000) Kangcheng Cat# KC-5G4; RRID: AB_2493106 Mouse anti-NeuN (IF, 1:500) Millipore Cat# MAB377; RRID: AB_2298772 Goat anti-chicken Alex 488 (IF, 1:400) Jackson ImmunoResearch Cat# 103-545-155; RRID: AB_2337390 Goat anti-rabbit Cy3 (IF, 1:400) Jackson ImmunoResearch Cat# 111-165-003; RRID: AB_2338000 Goat anti-rabbit HRP (WB, 1:12,000) Multi Science Cat# GAR0072; RRID:AB_2827833 Goat anti-mouse Alexa 488 (IF, 1:400) Jackson ImmunoResearch Cat#115-545-003; RRID: AB_2338840 Goat anti-mouse HRP (WB, 1:15,000) Multi Science Cat# GAM0072; RRID: AB_2827834 Bacterial and virus strains AAV-CAG-shBest1-eGFP Obio Technology custom-made AAV-CAG-shScrambled-eGFP Obio Technology custom-made AAV-Syn-Cre-eGFP Obio Technology Cat# H4942 AAV-Syn-eGFP Obio Technology Cat# E1190 AAV-CaMKIIa-GCaMP6s BrainVTA Cat# PT-0110 AAV-CaMKIIα-stargazin(C302S)-3flagT2A-mCherry GeneChem custom-made AAV-CaMKIIα-3flag-T2A-mCherry GeneChem NA AAV-Syn-taCasp3-eGFP BrainVTA Cat# PT-2433 AAV-Syn-eGFP BrainVTA Cat# PT-1990 Chemicals, peptides, and recombinant proteins Isoflurane RWD Life Science Cat# R510-220 Dihydrokainic acid (DHK) Tocris Cat# 0111 Ceftriaxone sodium (CTX) Roche NA L-glutamic acid (glutamate) Sigma-Aldrich Cat# G1251 Hoechst 332588 (HOE) Sigma-Aldrich Cat# B1155 Bicuculline methobromide (BMI) Tocris Cat# 0109 Kynurenic acid (KynA) Sigma-Aldrich Cat# K3375 Sulfasalazine (SAS) Sigma-Aldrich Cat# PHR3243 DL-threo-β-benzyloxyaspartic acid (TBOA) Tocris Cat# 1223 Tetrodotoxin (TTX) Sigma-Aldrich Cat# T8024 D-(− )-2-Amino-5-phosphonopentanoic acid (AP-5) Tocris Cat# 0106 (Continued on next page) 20 Cell Reports 45, 117229, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER 6-cyano-7-nitroquinoxaline-2,3dioneis (CNQX) Sigma-Aldrich Cat# C239 CaCCinh-A01 Tocris Cat# 4877 DCPIB Tocris Cat# 1540 T16Ainh-A01 Tocris Cat# 4538 1,2-bis(2-aminophenoxy)ethaneN,N,N,N-tetraacetic acid (BAPTA) Sigma-Aldrich Cat# A4926 4,4′-Diisothio-cyanostilbene-2,2′disulfonic acid (DIDS) Tocris Cat# 4523 Dil Beyotime Biotechnology Cat# C1036 TEA chloride Sigma-Aldrich Cat# T2265 Critical commercial assays FD rapid GolgiStain kit FD Neuro Technologies Cat# PK401 Deposited data Original western blot images This paper MendeleyData, https://doi.org/10.17632/ 9knfvskjfz.1 Experimental models: Organisms/strains C57BL/6 mice GemPharmatech Co. Ltd Cat# N000013 BestloxP/loxP (C57BL/6JGpt-Best1em1Cflox/ Gpt) mice GemPharmatech Co. Ltd Strain# T017974; RRID: IMSR_GPT:T017974 nNOSloxP/loxP mice A gift from Prof. Qi-Gang Zhou (Nanjing Medical University, China) NA Software and algorithms Adobe Photoshop CS6 Adobe Systems https://www.adobe.com/; RRID: SCR_014199 GraphPad Prism 8.0.2 GraphPad Software, Inc. https://www.graphpad.com/; RRID: SCR_002798 Image-Pro Plus software Media Cybernetics http://www.mediacy.com/imageproplus; RRID:SCR_007369 Imaris 7.5.2 software Oxford Instruments https://imaris.oxinst.cn/products/imaris- for-neuroscientists/; RRID: SCR_007370 pClamp 10.3 Molecular Devices http://mdc.custhelp.com/; RRID: SCR_011323 Mini analysis program 6.0 Synaptosoft, Inc. http://www.synaptosoft.com/ MiniAnalysis/; RRID: SCR_002184 Image Lab (Beta 7) Bio-Rad Laboratories http://www.bio-rad.com/en-us/sku/ 1709690-image-lab-software/; RRID: SCR_014210 ZEN 3.4 Light Edition Zeiss https://www.zeiss.com/; RRID: SCR_ 023747 MATLAB Thinker Tech Nanjing Biotech Ltd. http://www.mathworks.com/products/ matlab/;RRID:SCR_001622 Analyst R914.6.2 AB Sciex https://sciex.com/products/software/ analyst-tf-software; RRID:SCR_015785 TopScan LITE software Clever Sys, Inc. http://www.cleversysinc.com/; RRID: SCR_014494 Python software Python Software Foundation http://www.python.org/; RRID:SCR_008394 Other Osmotic micropump Alzet Model 1007D Enhanced chemiluminescence Millipore Cat #WBKLS0500 Cell Reports 45, 117229, April 28, 2026 21 ..



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    diisothio cyanostilbene  (Tocris)
    94
    Tocris diisothio cyanostilbene
    Diisothio Cyanostilbene, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/diisothio+cyanostilbene/DIDS/pm41955096-358-44-54
    Average 94 stars, based on 1 article reviews
    diisothio cyanostilbene - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier

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